Solvent Systems for Silica-gel Column Chromatography of Indole Derivatives

Separation of lipids by Silica Gel G column chromatography.

A column chromatographic procedure utilizing Silica Gel C is described for separating lipid components of serum and lipoproteins into individual fractions containing hydrocarbons (I), cholesterol esters (II), triglycerides (111), cholesterol (IV) , free fatty acids (V) , and phospholipids (VI). Silica Gel G required no pretreatment except adjustment of moisture content to 10%. The method afford...

Separation and Quantification of Hydrocarbons of LPG Using Novel MWCNT-Silica Gel Nanocomposite as Packed Column Adsorbent of Gas Chromatography

In this study, a new silica gel based adsorbent was fabricated and its ability in separation and quantification of alkanes mixture was investigated. Silica gel (SiO2) is a polar absorbent which is mainly used to separate polar compounds. Also, the carbon materials such as activated carbon and recently carbon nanotube (CNTs), have been widely used for separation of nonpolar materials. Carbon nan...

New silica gel-based monolithic column for nano-liquid chromatography, used in the HILIC mode.

This paper describes the synthesis and chromatographic and morphologic characterization of two monolithic silica nano-columns (50 µm i.d.) prepared by sol-gel processes, using hydrophilic interaction (HILIC) mode separations to evaluate their performance. Two types of monoliths were prepared by varying the precursors (tetraethoxysilane or a tetraethoxysilane-methyltrimethoxysilane mixture) and ...

Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography.

Rat plasma, containing 125I-labeled triglyceride-rich lipoprotein, was mixed following lipid extraction with 10% SDS buffer and analyzed by gel filtration chromatography on columns using an elution buffer containing 1% SDS. Labeled apoproteins were separated into apo B, apo E, and apo C radioactivity peaks. Labeled peptides, tyrosine, and iodide were also resolved by this method. Isolated lipop...

Surfactant Enhanced Chromium Removal Using a Silica Gel Column